Vitamin & Mineral Testing
Vitamins and minerals are the small building blocks the body relies on for energy, nerve signaling, blood formation, bone health, and countless everyday processes. Vitamin and mineral lab tests measure these nutrients, or the markers connected to them, in the blood โ and clinicians commonly use them to help evaluate symptoms like fatigue, low energy, tingling, or general questions about nutritional status.
What these tests measure
Vitamin and mineral testing looks at the nutrients circulating in your blood, or at related markers that reflect how much of a nutrient is available for the body to use. Some tests measure a vitamin directly, while others measure a substance that rises or falls depending on how well a particular vitamin is doing its job. Both approaches give a clinician information about nutritional status.
Because these nutrients take part in so many different systems, no single result tells the whole story. A clinician often reads several markers together, along with your symptoms and history, rather than reacting to one value alone. Most of this testing is done from an ordinary blood sample, so a single draw can cover one nutrient or several at once, depending on what has been ordered.
The core vitamin and mineral markers
Vitamin D, usually measured as 25-hydroxyvitamin D, is the most common marker of vitamin D status. It reflects the form of vitamin D that circulates in the blood and is a frequent starting point for people asking about bone health, energy, or general nutrition.
Vitamin B12 and folate are two B vitamins that are often considered together, since both take part in the healthy formation of blood cells and in nerve function. When a clinician is exploring these, methylmalonic acid, or MMA, is sometimes added โ it is a marker that can help reflect B12 activity at the tissue level rather than only what is circulating.
Magnesium is a mineral involved in muscle and nerve function, energy processes, and many enzyme reactions throughout the body. It is one of several minerals people ask about, alongside markers connected to iron and other trace minerals, when they want a broader read on nutritional status.
Why people test
People consider vitamin and mineral testing for many reasons. Some have lingering symptoms like tiredness, low energy, or tingling that they want to explore with a clinician. Others follow eating patterns, take certain medications, or have digestive conditions that raise questions about how well nutrients are absorbed.
Testing is also part of routine curiosity about wellness โ a way to gather information and bring it to a clinician. Whatever the reason, a lab result is a data point for a conversation, not a self-diagnosis. A clinician is best placed to say whether a marker is worth checking and what, if anything, it suggests in the context of your health.
What to expect when testing
Vitamin and mineral testing uses a standard blood draw. After ordering online, you visit a patient service center to have the sample collected โ typically without an appointment โ and a phlebotomist handles the draw, which takes only a few minutes.
Preparation can vary by test and by lab, so it is worth reading the instructions for whatever you order, since some tests suggest fasting and others do not. If you take supplements or medications, it is worth mentioning them to your clinician, since the timing of a dose relative to the draw can sometimes matter. Results are posted to your account once the lab has processed the sample, so you can view them privately and share them with a clinician.
Making sense of results
A vitamin or mineral result is a snapshot, not a verdict. Levels can shift with recent meals, supplements, illness, hydration, and other everyday factors, which is why context matters so much. A clinician reads results against your symptoms, history, diet, and any medications before drawing conclusions, and this page does not interpret what any particular value means.
No single nutrient test establishes a diagnosis on its own, and none of this information is a guide to how much of any vitamin or mineral to take. Decisions about supplements or diet belong with a clinician who can weigh the full picture. Reading results as information to discuss โ rather than an answer in themselves โ is the most reliable way to make use of them.
Vitamins & Minerals tests
Vitamin D 25 Hydroxy (Gold Standard)
Lab test
Coenzyme Q10
Lab test
Copper
Lab test
Folate
Lab test
Vitamin B1 (Thiamine)
Lab test
Magnesium
Lab test
Vitamin B6
Lab test
Vitamin B12
Lab test
Vitamin D (D2,D3) QuestAssureD 25-OH
Lab test
Vitamin C
Lab test
Vitamin E
Lab test
Zinc
Lab test
The vitamins & minerals bundle
Vitamin D, B12, iron, and other nutrient levels.
Recent vitamins & minerals research
Recent peer-reviewed papers from the medical literature, via PubMed. Listed for reference โ not medical advice.
Frequently asked questions
What do vitamin and mineral tests measure?+โ
They measure nutrients circulating in the blood, or related markers that reflect how a nutrient is being used. Clinicians use them to help evaluate nutritional status alongside symptoms and history, rather than as stand-alone answers.
Which vitamin and mineral tests do people order most?+โ
Vitamin D and vitamin B12 are among the most commonly ordered, often alongside folate and minerals such as magnesium. Many people talk through which markers make sense for them with their clinician.
Do I need to fast before vitamin or mineral testing?+โ
It depends on the specific test and lab. Some suggest fasting and others do not, so it is worth checking the instructions for what you order and mentioning any supplements to your clinician, since timing can matter.
Can a vitamin test tell me how much to supplement?+โ
No. These pages and tests do not provide dosing guidance. A result is one piece of information a clinician weighs with your history, diet, and symptoms before making any recommendation about supplements.
Can a lab test diagnose a vitamin deficiency?+โ
No single lab test diagnoses a deficiency on its own. Results are one input a clinician considers together with your symptoms, history, and sometimes additional markers before reaching any conclusion.