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Lab test

Varicella-Zoster Virus Antibody (Immunity Screen), ACIF Test

This test measures varicella-zoster virus antibody in a blood sample using anti-complement immunofluorescence, or ACIF. Clinicians use it to look for evidence of past infection or vaccination, or to evaluate susceptibility to chickenpox when antibody is absent.

Blood drawQuest patient service center

About this test

Varicella-zoster virus is the virus behind chickenpox and shingles. This test measures antibody to the virus using the ACIF method, a laboratory technique distinct from other varicella antibody assays.

The result is read as either evidence that antibody is present, which points to past infection or vaccination, or that it is absent, which can be used as evidence of susceptibility to varicella. A clinician interprets the result in the context of a person's history and the reason the test was ordered.

Test details

Quest code14505
MethodologyAnti-Complement Immunofluorescence
Sample type1 mL serum
CollectionQuest patient service center

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Quest resources

Additional details

What this test looks at

The test reports a single analyte: varicella-zoster virus antibody, detected using the anti-complement immunofluorescence method. This immunity screen is a different assay from a standard varicella IgG antibody test, though both look at antibody to the same virus.

Order online and a physician order is included — no doctor's visit and no insurance needed. Your sample is collected at a Quest Diagnostics patient service center.

Laboratory results should always be interpreted by a licensed clinician who knows your health history. This page is information about a test, not medical advice.

Frequently asked questions

What does this test measure?

It measures antibody to varicella-zoster virus using the anti-complement immunofluorescence (ACIF) method, to check for evidence of past infection or vaccination.

How is the sample collected?

The test uses a 1 mL serum sample from a routine blood draw.

Do I need to prepare for this test?

No preparation instructions are listed for this test, though people follow any instructions their ordering clinician provides.

What does it mean if antibody is not detected?

Quest describes the absence of detectable antibody as evidence of susceptibility to varicella (chickenpox). A clinician reads this alongside a person's history.

How soon does antibody appear after infection?

Quest describes antibody as usually detectable within about two days after a rash appears, and it remains detectable for life once present.

How much is a Varicella-Zoster Virus Antibody , ACIF test?

$239, paid once at checkout. The price includes the physician's order this test requires and sample collection at a Quest patient service center.

Last updated 2026-09-18 · How we write about lab testing

Educational content

This page is for general educational purposes only. It is not medical advice, and it is not a substitute for consultation with a qualified healthcare professional. Laboratory results should always be interpreted by a licensed clinician who knows your health history. If you have questions about your health or your results, talk with your doctor. If you think you may be experiencing a medical emergency, call 911.