RPR with Reflex to Titer and Treponemal Antibody Test
This test uses Quest's traditional syphilis testing algorithm: an RPR (Rapid Plasma Reagin) screen on serum, with reflex to a titer and to a Treponema pallidum antibody immunoassay when the screen is reactive.
About this test
This is a two-step syphilis test using the traditional algorithm. The first step is an RPR flocculation test, a nontreponemal test that screens a 3 mL serum sample for reactivity.
Quest lists this product under several names, including Syphilis Serology Screen, Premarital RPR, Automated Reagin Test (ART) and Rapid Plasma Reagin — they describe the same traditional testing sequence.
Who might consider this test?
- Quest describes this testing as appropriate for people with signs or symptoms suggestive of syphilis
- For screening asymptomatic individuals
- Including certain populations at increased risk for infection and pregnant women
Test details
| Collection | Quest patient service center |
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Quest resources
Additional details
What this test looks at
The first stage measures nontreponemal (lipoidal antigen) antibody reactivity using RPR flocculation on a 3 mL serum sample. A reactive result is automatically reflexed to a titer and to a Treponema pallidum antibody immunoassay, following what Quest calls the traditional testing algorithm.
How it's often used alongside other tests
Because a treponemal test stays positive long after treatment, Quest notes that a single test type is not enough on its own; confirmation with the treponemal-specific assay is part of the same order. A clinician reads the combined result — nontreponemal screen, titer where applicable, and treponemal confirmation — together with history and symptoms.
This test isn't part of the Celio Health store at the moment, so it can't be ordered or requested here. The test menu lists everything that can.
Laboratory results should always be interpreted by a licensed clinician who knows your health history. This page is information about a test, not medical advice.
Frequently asked questions
What does this test measure?
It first measures nontreponemal (lipoidal antigen) antibodies using RPR; a reactive result is reflexed to a titer and to a Treponema pallidum antibody immunoassay, following Quest's traditional testing algorithm.
How is the sample collected?
Through a routine blood draw; the test uses a 3 mL serum sample.
Do I need to prepare for this test?
No preparation instructions are listed for this test, though people follow any instructions their ordering clinician provides.
Is this test also called something else?
Yes. Quest lists it under several names, including Syphilis Serology Screen, Premarital RPR, Automated Reagin Test (ART) and Rapid Plasma Reagin.
Can a reactive RPR result be a false positive?
Quest notes that nontreponemal test results can be falsely positive in a range of conditions, including autoimmune disease, cancer, other infections, older age and pregnancy, which is part of why a reactive screen is followed by a treponemal-specific confirmatory test.