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Lab test

Heterophile Antibody (Mono Screen) Test

This test measures heterophile antibodies in a blood sample, a marker Quest associates with infectious mononucleosis, caused by the Epstein-Barr virus.

Quest patient service center

About this test

Heterophile, Mono Screen looks for heterophile antibodies in a 1 mL serum sample using an agglutination method. Quest describes these antibodies as associated with infectious mononucleosis, an illness caused by the Epstein-Barr virus.

Mono Screen looks at one marker only. Quest's description ties heterophile antibody presence specifically to infectious mononucleosis, so a positive or negative result is read together with the timing of symptoms and, when needed, other findings, rather than standing alone.

Test details

CollectionQuest patient service center

Quest resources

Additional details

What this test looks at

The test reports the presence or absence of heterophile antibodies in a 1 mL serum sample, using an agglutination method. Quest also lists this product under the names Mono Screen and Infectious Mononucleosis, all referring to the same measurement.

This test isn't part of the Celio Health store at the moment, so it can't be ordered or requested here. The test menu lists everything that can.

Laboratory results should always be interpreted by a licensed clinician who knows your health history. This page is information about a test, not medical advice.

Frequently asked questions

What does this test measure?

It measures heterophile antibodies in a serum sample, using an agglutination method. Quest associates these antibodies with infectious mononucleosis.

How is the sample collected?

The test uses a 1 mL serum sample from a routine blood draw.

Do I need to prepare for this test?

No preparation instructions are listed for this test, though people follow any instructions their ordering clinician provides.

How soon can this test detect mononucleosis?

Quest notes heterophile antibodies may appear as early as the fourth day of illness, with about 90% of people testing positive by the twenty-first day.

Is "Mono Screen" the same test?

Yes. Quest lists this product under the names Mono Screen and Infectious Mononucleosis; they refer to the same heterophile antibody measurement.

Last updated 2026-09-18 · How we write about lab testing

Educational content

This page is for general educational purposes only. It is not medical advice, and it is not a substitute for consultation with a qualified healthcare professional. Laboratory results should always be interpreted by a licensed clinician who knows your health history. If you have questions about your health or your results, talk with your doctor. If you think you may be experiencing a medical emergency, call 911.