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Lab test

Diphtheria and Tetanus Antitoxoids Test

This test measures antibody levels to diphtheria and tetanus toxoids in a blood sample. Clinicians use it to help evaluate a person's immune response to diphtheria and tetanus immunization, including after booster doses.

Quest patient service center

About this test

Diphtheria and tetanus antitoxoid antibodies are measured from two separate serum samples using an immunoassay method. The test reports antibody levels for each toxoid separately, which is how a clinician can look at a person's immune response to each vaccine component individually rather than as a single combined result.

Quest notes that tetanus, caused by the bacterium Clostridium tetani, can still occur in some individuals even when antibody levels are considered protective, and that these results are not intended to determine whether antitoxin should be given when it is clinically indicated. That caveat is part of why the result is read by a clinician in context rather than treated as a stand-alone answer.

Who might consider this test?

  • It comes up when a clinician wants to check how a person's antibody levels have responded to prior vaccination.

Test details

CollectionQuest patient service center

Quest resources

Additional details

What this test looks at

The test reports antibody levels against diphtheria antitoxoid and against tetanus antitoxoid, from two separate serum samples analyzed by immunoassay. Because each toxoid is reported separately, a clinician can see how a person's immune response differs between the two vaccine components.

This test isn't part of the Celio Health store at the moment, so it can't be ordered or requested here. The test menu lists everything that can.

Laboratory results should always be interpreted by a licensed clinician who knows your health history. This page is information about a test, not medical advice.

Frequently asked questions

What does this test measure?

It measures antibody levels against diphtheria toxoid and against tetanus toxoid in blood, used to evaluate a person's immune response to these vaccines.

How is the sample collected?

The test uses two separate serum samples from a routine blood draw, analyzed using an immunoassay method.

Do I need to prepare for this test?

No preparation instructions are listed for this test, though people follow any instructions their ordering clinician provides.

Why might a clinician order this test?

It is used to evaluate a person's immune response to diphtheria immunization and to tetanus toxoid immunization, including after booster doses, in people with a typically functioning immune system.

Does a protective-range result mean tetanus can't occur?

No. Quest notes that tetanus can still occur in some individuals even with antibody levels considered protective, and that these results are not intended to determine whether antitoxin should be given when clinically indicated. A clinician weighs the result alongside the clinical picture.

Last updated 2026-09-18 · How we write about lab testing

Educational content

This page is for general educational purposes only. It is not medical advice, and it is not a substitute for consultation with a qualified healthcare professional. Laboratory results should always be interpreted by a licensed clinician who knows your health history. If you have questions about your health or your results, talk with your doctor. If you think you may be experiencing a medical emergency, call 911.