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Lab test

Jo-1 Antibody Test

This test measures Jo-1 antibody in a blood sample, an antibody Quest associates with polymyositis and related conditions.

Quest patient service center

About this test

Jo-1 antibody is found most often in polymyositis, present in about 31% of cases according to Quest, and has also been found in dermatomyositis and in overlap syndromes that combine polymyositis with scleroderma or with systemic lupus erythematosus.

The test is performed on a 1 mL serum sample using immunoassay methodology. Because Jo-1 antibody is associated with more than one condition, a positive result is read by a clinician alongside symptoms, other autoantibody testing and clinical findings rather than as a stand-alone diagnosis.

Who might consider this test?

  • Usually being evaluated for polymyositis
  • An overlap syndrome involving these connective tissue conditions
  • Often because of muscle-related symptoms a clinician is investigating

Test details

CollectionQuest patient service center

Quest resources

Additional details

What this test looks at

The test reports the presence of Jo-1 antibody in serum. Quest ties this antibody most closely to polymyositis, while also noting it can appear in dermatomyositis and in overlap syndromes with scleroderma or systemic lupus erythematosus.

This test isn't part of the Celio Health store at the moment, so it can't be ordered or requested here. The test menu lists everything that can.

Laboratory results should always be interpreted by a licensed clinician who knows your health history. This page is information about a test, not medical advice.

Frequently asked questions

What does this test measure?

It measures Jo-1 antibody in a serum sample, using immunoassay methodology.

How is the sample collected?

The test uses a 1 mL serum sample from a routine blood draw.

Do I need to prepare for this test?

No preparation instructions are listed for this test, though people follow any instructions their ordering clinician provides.

What conditions is Jo-1 antibody associated with?

Quest associates it most often with polymyositis, present in about 31% of cases, and also notes it can appear in dermatomyositis and in overlap syndromes with scleroderma or systemic lupus erythematosus.

Does a positive result diagnose polymyositis?

No. A positive result is read by a clinician alongside symptoms, other testing and clinical findings, not as a stand-alone diagnosis.

Last updated 2026-09-18 · How we write about lab testing

Educational content

This page is for general educational purposes only. It is not medical advice, and it is not a substitute for consultation with a qualified healthcare professional. Laboratory results should always be interpreted by a licensed clinician who knows your health history. If you have questions about your health or your results, talk with your doctor. If you think you may be experiencing a medical emergency, call 911.