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Lab test

ANCA Screen with MPO and PR3, with Reflex to ANCA Titer Test

This test measures antineutrophil cytoplasmic antibodies (ANCA), including P-ANCA and C-ANCA, along with myeloperoxidase and proteinase-3 antibodies, in a blood sample. A positive screen reflexes to ANCA titer testing.

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About this test

The panel measures antineutrophil cytoplasmic antibodies in serum, along with the two specific antibodies most often associated with them, myeloperoxidase antibody and proteinase-3 antibody. Quest describes these antibodies as having been associated with certain vascular diseases, inflammatory bowel disease, and other autoimmune diseases, and notes that testing for them has been found useful in establishing a diagnosis in those areas.

If the ANCA screen is positive, the sample reflexes to C-ANCA titer, P-ANCA titer, or atypical P-ANCA titer as applicable, reported alongside the original screen and the MPO and PR3 antibody results.

Who might consider this test?

  • Typically being evaluated by a clinician for a suspected vascular disease
  • Inflammatory bowel disease
  • Other autoimmune disease where ANCA testing has been found useful in establishing a diagnosis

Test details

CollectionQuest patient service center

Quest resources

Additional details

What this test looks at

The panel reports three analytes from the same serum sample: the ANCA screen itself, the myeloperoxidase antibody level, and the proteinase-3 antibody level. Quest lists immunoassay as the methodology used to measure all three.

This test isn't part of the Celio Health store at the moment, so it can't be ordered or requested here. The test menu lists everything that can.

Laboratory results should always be interpreted by a licensed clinician who knows your health history. This page is information about a test, not medical advice.

Frequently asked questions

What does the ANCA Screen with MPO and PR3 measure?

It measures antineutrophil cytoplasmic antibodies (ANCA) in serum, along with myeloperoxidase antibody and proteinase-3 antibody, using immunoassay.

How is the sample collected?

The test uses a 2 mL serum sample from a routine blood draw.

Do I need to prepare for this test?

No preparation instructions are listed for this test, though people follow any instructions their ordering clinician provides.

What happens if the ANCA Screen is positive?

A positive screen reflexes to ANCA titer testing, which may include C-ANCA titer, P-ANCA titer, or atypical P-ANCA titer.

Last updated 2026-09-18 · How we write about lab testing

Educational content

This page is for general educational purposes only. It is not medical advice, and it is not a substitute for consultation with a qualified healthcare professional. Laboratory results should always be interpreted by a licensed clinician who knows your health history. If you have questions about your health or your results, talk with your doctor. If you think you may be experiencing a medical emergency, call 911.