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Lab test

TSH Antibody Test

This test measures TSH autoantibodies in a blood sample. It is described as useful when a serum TSH level appears discordant with clinical findings or other thyroid test results.

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About this test

TSH autoantibodies can bind TSH to form a larger molecule known as macro-TSH, which has low biological activity but can interfere with standard TSH immunoassays. The test is measured by radioimmunoassay on a serum sample, and a positive result offers one possible explanation when a TSH value does not match the clinical picture or other thyroid results, such as a markedly elevated TSH alongside normal free triiodothyronine and free thyroxine levels.

Test details

CollectionQuest patient service center

Quest resources

Additional details

What this test looks at

The test reports one value: the level of TSH autoantibody activity in serum. Because these antibodies can interfere with routine TSH assays, a clinician reads this result alongside pertinent history, physical examination findings and other thyroid test results, since other factors, such as interfering substances or subclinical hypothyroidism, can also produce a discordant TSH.

This test isn't part of the Celio Health store at the moment, so it can't be ordered or requested here. The test menu lists everything that can.

Laboratory results should always be interpreted by a licensed clinician who knows your health history. This page is information about a test, not medical advice.

Frequently asked questions

What does this test measure?

It measures TSH autoantibody activity in a serum sample, using radioimmunoassay.

How is the sample collected?

The test uses a serum sample from a routine blood draw.

Do I need to prepare for this test?

No preparation instructions are listed for this test, though people follow any instructions their ordering clinician provides.

When is this test used?

It may be used when a serum TSH level is discordant with clinical findings or other thyroid results, to help determine whether TSH autoantibodies are a contributing factor.

What is macro-TSH?

Macro-TSH is a larger molecule formed when TSH autoantibodies bind TSH. It has low biological activity but can interfere with how standard TSH immunoassays read a sample.

Last updated 2026-09-18 · How we write about lab testing

Educational content

This page is for general educational purposes only. It is not medical advice, and it is not a substitute for consultation with a qualified healthcare professional. Laboratory results should always be interpreted by a licensed clinician who knows your health history. If you have questions about your health or your results, talk with your doctor. If you think you may be experiencing a medical emergency, call 911.