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Lab test

Cortisol, Total Test

The total cortisol test measures the total amount of cortisol, a hormone, in a blood sample. Quest lists it under names including stress hormone, adrenal cortisol, hydrocortisone and total cortisol.

Blood drawQuest patient service center

About this test

Cortisol is a hormone produced by the adrenal glands. This test reports the total concentration of cortisol in a serum sample, analyzed using an immunoassay.

Because cortisol naturally varies through the day and can be affected by many factors, a single result is read by a clinician together with symptoms, history and the reason the test was ordered, rather than as a stand-alone answer.

Test details

Quest code367
MethodologyImmunoassay
Sample type1 mL serum
CollectionQuest patient service center

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Quest resources

Additional details

What this test looks at

The test reports one value: the total concentration of cortisol in a serum sample, measured using immunoassay. Quest lists it under several names, including stress hormone, adrenal cortisol, hydrocortisone and total cortisol, all referring to the same measurement.

Order online and a physician order is included — no doctor's visit and no insurance needed. Your sample is collected at a Quest Diagnostics patient service center.

Laboratory results should always be interpreted by a licensed clinician who knows your health history. This page is information about a test, not medical advice.

Frequently asked questions

What does the total cortisol test measure?

It measures the total amount of cortisol, a hormone produced by the adrenal glands, in a serum sample using immunoassay.

How is the sample collected?

The test uses a serum sample from a routine blood draw at a Quest Diagnostics patient service center.

Do I need to prepare for a cortisol test?

No preparation instructions are listed for this test, though people follow any instructions their ordering clinician provides.

Why might a clinician order a cortisol test?

Quest describes cortisol as increased in Cushing's Disease and decreased in Addison's Disease, and elevated cortisol has also been associated with difficult-to-control type 2 diabetes. A clinician orders the test to help evaluate one of these possibilities, alongside other findings.

Is this the same as a salivary or urine cortisol test?

No. This is a blood test measuring total cortisol in serum. Saliva- or urine-based cortisol tests are separate products with their own collection methods.

What does a Cortisol Total test cost without insurance?

$35. Celio Health's price is a flat cash price and insurance is never billed: the required physician's order is included.

Last updated 2026-09-18 · How we write about lab testing

Educational content

This page is for general educational purposes only. It is not medical advice, and it is not a substitute for consultation with a qualified healthcare professional. Laboratory results should always be interpreted by a licensed clinician who knows your health history. If you have questions about your health or your results, talk with your doctor. If you think you may be experiencing a medical emergency, call 911.