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Lab test

17-Hydroxypregnenolone Test

The 17-hydroxypregnenolone test measures a steroid hormone precursor in a blood serum sample. It is described as a test used in evaluating certain rare enzyme deficiencies linked to congenital adrenal hyperplasia.

Quest patient service center

About this test

17-Hydroxypregnenolone is a steroid hormone precursor. Quest describes this test as measuring its level in a blood serum sample, and the result is used in evaluating 3-Beta-Hydroxylase enzyme deficiency, a rare cause of congenital adrenal hyperplasia, and 17-Hydroxylase (P450c17) enzyme deficiency.

The sample is a 2 mL serum specimen, collected in a red-top tube without gel, and analyzed using chromatography/mass spectrometry. Because the conditions it helps evaluate are rare, this test is typically ordered by a clinician working through a specific differential rather than as part of routine screening.

Who might consider this test?

  • It is not a general screening test.

Test details

CollectionQuest patient service center

Quest resources

Additional details

What this test looks at

The test reports the level of 17-hydroxypregnenolone, a steroid hormone precursor, in blood serum. Quest lists no alternate aliases for this product, and the sample is analyzed using chromatography/mass spectrometry.

This test isn't part of the Celio Health store at the moment, so it can't be ordered or requested here. The test menu lists everything that can.

Laboratory results should always be interpreted by a licensed clinician who knows your health history. This page is information about a test, not medical advice.

Frequently asked questions

What does the 17-hydroxypregnenolone test measure?

It measures the level of 17-hydroxypregnenolone, a steroid hormone precursor, in a blood serum sample, using chromatography/mass spectrometry.

How is the sample collected?

The test uses a blood draw, collected as a 2 mL serum sample in a red-top tube without gel.

Do I need to prepare for this test?

No preparation instructions are listed for this test, though people follow any instructions their ordering clinician provides.

What is this test used to evaluate?

It is used in evaluating 3-Beta-Hydroxylase enzyme deficiency, a rare cause of congenital adrenal hyperplasia, and 17-Hydroxylase (P450c17) enzyme deficiency.

Last updated 2026-09-18 · How we write about lab testing

Educational content

This page is for general educational purposes only. It is not medical advice, and it is not a substitute for consultation with a qualified healthcare professional. Laboratory results should always be interpreted by a licensed clinician who knows your health history. If you have questions about your health or your results, talk with your doctor. If you think you may be experiencing a medical emergency, call 911.