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Lab test

TMAO (Trimethylamine N-Oxide) Test

The TMAO test measures trimethylamine N-oxide in a blood sample. TMAO is a metabolite produced by gut bacteria and is described as related to processes involved in the development of atherosclerosis.

Quest patient service center

About this test

TMAO is a compound produced when gut bacteria act on nutrients such as phosphatidylcholine, choline and L-carnitine, found in foods including red meat, egg yolk and full-fat dairy. The liver converts the intermediate compound gut bacteria produce, TMA, into TMAO, which the test then measures directly from a serum sample.

TMAO has been described as regulating physiological processes involved in the development of atherosclerosis, a condition related to plaque buildup in arteries. Because it reflects both diet and gut bacteria activity, a clinician reads a TMAO result alongside a person's broader cardiovascular risk picture rather than as a standalone finding.

Test details

CollectionQuest patient service center

Quest resources

Additional details

What this test looks at

The test reports one value: the concentration of TMAO in serum, measured using liquid chromatography/tandem mass spectrometry. Quest notes that fasting overnight and avoiding fish or other seafood the day before the blood draw helps avoid false elevations in the result, since seafood itself is a dietary source of compounds related to TMAO.

This test isn't part of the Celio Health store at the moment, so it can't be ordered or requested here. The test menu lists everything that can.

Laboratory results should always be interpreted by a licensed clinician who knows your health history. This page is information about a test, not medical advice.

Frequently asked questions

What does the TMAO test measure?

It measures the concentration of trimethylamine N-oxide (TMAO) in a serum sample, using liquid chromatography/tandem mass spectrometry.

How is the TMAO sample collected?

The test uses a serum sample from a routine blood draw.

Do I need to prepare for a TMAO test?

Quest lists preparation for this test, including fasting overnight and avoiding fish or other seafood the day before the blood draw; preparation details appear with the test details.

What produces TMAO in the body?

Gut bacteria act on nutrients such as choline, phosphatidylcholine and L-carnitine, found in foods like red meat, egg yolk and full-fat dairy, and the liver converts the resulting compound into TMAO.

Does a TMAO result diagnose heart disease?

No. It is described as related to processes involved in atherosclerosis, and a clinician reads it together with other cardiovascular risk information rather than as a diagnosis on its own.

Last updated 2026-09-18 · How we write about lab testing

Educational content

This page is for general educational purposes only. It is not medical advice, and it is not a substitute for consultation with a qualified healthcare professional. Laboratory results should always be interpreted by a licensed clinician who knows your health history. If you have questions about your health or your results, talk with your doctor. If you think you may be experiencing a medical emergency, call 911.