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Lab test

Hepatitis B Surface Antigen (HBsAg) with Reflex Confirmation Test

This test measures Hepatitis B Surface Antigen (HBsAg) in a serum sample. If the result is positive, the laboratory automatically runs a confirmatory test based on the manufacturer's FDA-approved recommendations.

Quest patient service center

About this test

Hepatitis B Surface Antigen usually appears in the blood one to six months after exposure to the Hepatitis B virus and tends to peak around the time symptoms begin. In most people it clears within one to three months.

The test is run on a 2 mL serum sample using an immunoassay method. Because a positive screening result on its own does not confirm infection, this product includes a reflex step: if the surface antigen result is positive, confirmatory testing is performed automatically on the same sample, following the test kit manufacturer's FDA-approved recommendations, rather than requiring a separate order.

Test details

CollectionQuest patient service center

Quest resources

Additional details

What this test looks at

The test reports two things: whether Hepatitis B Surface Antigen is present, and — only if that first result is positive — the outcome of a confirmatory test run automatically on the same sample. This reflex step means a positive screen does not require a second, separate order.

This test isn't part of the Celio Health store at the moment, so it can't be ordered or requested here. The test menu lists everything that can.

Laboratory results should always be interpreted by a licensed clinician who knows your health history. This page is information about a test, not medical advice.

Frequently asked questions

What does this test measure?

It measures Hepatitis B Surface Antigen (HBsAg) in a serum sample, an antigen that typically appears one to six months after exposure to Hepatitis B virus and peaks around the onset of symptoms.

How is the sample collected?

The test uses a 2 mL serum sample from a routine blood draw at a Quest Diagnostics patient service center.

Do I need to prepare for this test?

No preparation instructions are listed for this test, though people follow any instructions their ordering clinician provides.

What happens if my HBsAg result is positive?

The laboratory automatically runs a confirmatory test on the same sample, following the manufacturer's FDA-approved recommendations, so a positive screen does not require a separate order.

Is HBsAg the same as "Hep B" or "Australian Antigen"?

Yes. Quest lists this test under several names, including Hep B, HBsAg and Australian Antigen; they all refer to the same Hepatitis B Surface Antigen measurement.

Last updated 2026-09-18 · How we write about lab testing

Educational content

This page is for general educational purposes only. It is not medical advice, and it is not a substitute for consultation with a qualified healthcare professional. Laboratory results should always be interpreted by a licensed clinician who knows your health history. If you have questions about your health or your results, talk with your doctor. If you think you may be experiencing a medical emergency, call 911.