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Lab test

Hepatitis B Surface Antibody, Qualitative Test

This test checks for hepatitis B surface antibody (anti-HBs) in a serum sample using immunoassay. Quest describes a detected result as indicating prior immunologic exposure to the hepatitis B antigen or vaccine.

Blood drawQuest patient service center

About this test

Hepatitis B surface antibody, qualitative, reports whether this antibody is detected in serum, rather than giving a numeric antibody level. Quest describes a positive result as indicating prior immunologic exposure to the hepatitis B antigen or the hepatitis B vaccine.

The test uses a 1 mL serum sample, analyzed by immunoassay. Because the result is reported as detected or not detected rather than as a value, it answers whether antibody is present without measuring how much.

Who might consider this test?

  • A clinician reads the result together with vaccination and exposure history.

Test details

Quest code499
MethodologyImmunoassay
Sample type1 mL serum
CollectionQuest patient service center

Reference range

Non-Reactive

Reference ranges are general guidance. The range printed on your own laboratory report is the one that applies to your result.

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Quest resources

Additional details

What this test looks at

The test reports whether hepatitis B surface antibody (anti-HBs) is present in a serum sample, using immunoassay. Quest describes a positive result as indicating prior immunologic exposure to the hepatitis B antigen or vaccine, without reporting a specific antibody amount.

Order online. No doctor's visit and no insurance needed. Your sample is collected at a Quest Diagnostics patient service center.

Laboratory results should always be interpreted by a licensed clinician who knows your health history. This page is information about a test, not medical advice.

Frequently asked questions

What does this test detect?

It detects hepatitis B surface antibody (anti-HBs) in a serum sample, indicating prior immunologic exposure to the hepatitis B antigen or vaccine.

How is the sample collected?

Through a routine blood draw, collected as a 1 mL serum sample.

Do I need to prepare for this test?

No preparation instructions are listed for this test. If a clinician has given you instructions, follow those.

Does this test tell me my exact antibody level?

No. This is a qualitative test, reporting whether hepatitis B surface antibody is detected rather than a numeric amount.

What does a detected result mean?

Quest describes a detected result as indicating prior immunologic exposure to the hepatitis B antigen or vaccine.

How much is a Hepatitis B Surface Antibody, Qualitative test?

The test is $89, plus $25 for the doctor's order and Quest blood draw. Total: $114. The $25 fee is waived at $149+. See how ordering works and our blood test cost guide.

Last updated 2026-10-30 · How we write about lab testing

Educational content

This page is for general educational purposes only. It is not medical advice, and it is not a substitute for consultation with a qualified healthcare professional. Laboratory results should always be interpreted by a licensed clinician who knows your health history. If you have questions about your health or your results, talk with your doctor. If you think you may be experiencing a medical emergency, call 911.