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Lab test

Hepatitis B Surface Antibody, Qualitative Test

This test checks for hepatitis B surface antibody (anti-HBs) in a serum sample using immunoassay. Quest describes a detected result as indicating prior immunologic exposure to the hepatitis B antigen or vaccine.

Blood drawQuest patient service center

About this test

Hepatitis B surface antibody, qualitative, reports whether this antibody is detected in serum, rather than giving a numeric antibody level. Quest describes a positive result as indicating prior immunologic exposure to the hepatitis B antigen or the hepatitis B vaccine.

The test uses a 1 mL serum sample, analyzed by immunoassay. Because the result is reported as detected or not detected rather than as a value, it answers whether antibody is present without measuring how much.

Who might consider this test?

  • A clinician reads the result together with vaccination and exposure history.

Test details

Quest code499
MethodologyImmunoassay
Sample type1 mL serum
CollectionQuest patient service center

Reference range

Non-Reactive

Reference ranges are general guidance. The range printed on your own laboratory report is the one that applies to your result.

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Quest resources

Additional details

What this test looks at

The test reports whether hepatitis B surface antibody (anti-HBs) is present in a serum sample, using immunoassay. Quest describes a positive result as indicating prior immunologic exposure to the hepatitis B antigen or vaccine, without reporting a specific antibody amount.

Order online and a physician order is included — no doctor's visit and no insurance needed. Your sample is collected at a Quest Diagnostics patient service center.

Laboratory results should always be interpreted by a licensed clinician who knows your health history. This page is information about a test, not medical advice.

Frequently asked questions

What does this test detect?

It detects hepatitis B surface antibody (anti-HBs) in a serum sample, indicating prior immunologic exposure to the hepatitis B antigen or vaccine.

How is the sample collected?

Through a routine blood draw, collected as a 1 mL serum sample.

Do I need to prepare for this test?

No preparation instructions are listed for this test, though people follow any instructions their ordering clinician provides.

Does this test tell me my exact antibody level?

No. This is a qualitative test, reporting whether hepatitis B surface antibody is detected rather than a numeric amount.

What does a detected result mean?

Quest describes a detected result as indicating prior immunologic exposure to the hepatitis B antigen or vaccine.

How much is a Hepatitis B Surface Antibody, Qualitative test?

$89, paid once at checkout. The price includes the physician's order this test requires and sample collection at a Quest patient service center.

Last updated 2026-09-18 · How we write about lab testing

Educational content

This page is for general educational purposes only. It is not medical advice, and it is not a substitute for consultation with a qualified healthcare professional. Laboratory results should always be interpreted by a licensed clinician who knows your health history. If you have questions about your health or your results, talk with your doctor. If you think you may be experiencing a medical emergency, call 911.