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Lab test

Carbon Dioxide (CO2) Test

The carbon dioxide test, also listed as CO2 content or bicarbonate, measures the level of carbon dioxide in a blood sample. It is a marker clinicians use in evaluating the body's acid-base balance, read together with other blood chemistry results.

Quest patient service center

About this test

Carbon dioxide in the blood is mostly present as bicarbonate, and Quest lists this same test under the names CO2 content, HCO3 and bicarbonate. The result is a single value that reflects the body's acid-base chemistry at the time of the draw, measured from a blood sample using spectrophotometry.

Quest describes this measurement as used in evaluating a range of conditions connected to changes in acid-base balance. Because a shift in this value can come from many different sources, a clinician reads it together with other basic chemistry results rather than by itself.

Test details

Quest code310
MethodologySpectrophotometry
Sample type1 full unopened spun gel barrier tube
CollectionQuest patient service center

Reference range

20-32 mmol/L

Reference ranges are general guidance. The range printed on your own laboratory report is the one that applies to your result.

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Quest resources

Additional details

What this test looks at

The test reports the concentration of carbon dioxide in the blood, which Quest also lists under the names CO2 content, HCO3 and bicarbonate — these all refer to the same measurement rather than separate tests.

Order online and a physician order is included — no doctor's visit and no insurance needed. Your sample is collected at a Quest Diagnostics patient service center.

Laboratory results should always be interpreted by a licensed clinician who knows your health history. This page is information about a test, not medical advice.

Frequently asked questions

What does this test measure?

It measures the concentration of carbon dioxide, mostly present as bicarbonate, in a blood sample analyzed by spectrophotometry.

How is the sample collected?

The test uses a blood sample collected in a specific tube (a full, unopened spun gel barrier tube), drawn at a Quest Diagnostics patient service center.

Do I need to prepare for this test?

No preparation instructions are listed for this test, though people follow any instructions their ordering clinician provides.

Is CO2 the same as bicarbonate?

Yes. Quest lists this test under several names — CO2 content, HCO3 and bicarbonate — and they all refer to the same carbon dioxide measurement.

What does a Carbon Dioxide test cost without insurance?

$15. Celio Health's price is a flat cash price and insurance is never billed: the required physician's order is included.

Last updated 2026-09-18 · How we write about lab testing

Educational content

This page is for general educational purposes only. It is not medical advice, and it is not a substitute for consultation with a qualified healthcare professional. Laboratory results should always be interpreted by a licensed clinician who knows your health history. If you have questions about your health or your results, talk with your doctor. If you think you may be experiencing a medical emergency, call 911.