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Lab test

Hemoglobin (HB) Test

The hemoglobin test measures the amount of hemoglobin, the oxygen-carrying protein in red blood cells, in a whole blood sample. It is commonly used as part of the evaluation for anemia and to calculate related red blood cell indices.

Blood drawQuest patient service center

About this test

Hemoglobin is the protein inside red blood cells that carries oxygen through the body. Quest describes this measurement as part of the evaluation for anemia, and it is also used to calculate other red blood cell indices that are often reported alongside it.

The test uses a whole blood sample, collected in an EDTA (lavender-top) tube, and is analyzed using electronic cell sizing and counting, cytometry, or microscopy. It reports a single value, which clinicians read together with other blood cell indices and a person's history and symptoms rather than by itself.

Who might consider this test?

  • People who talk with their clinician about a hemoglobin test are often being evaluated for anemia or having a related blood cell index calculated.

Test details

Quest code510
MethodologyElectronic Cell Sizing and Counting/Cytometry/Microscopy
Sample typeWhole blood
CollectionQuest patient service center

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Quest resources

Additional details

What this test looks at

The test reports one value: the amount of hemoglobin in a whole blood sample. Quest also lists it under the abbreviations HB and Hgb. Hemoglobin is the protein in red blood cells responsible for carrying oxygen, and this measurement is commonly used in the evaluation of anemia.

How it's often used alongside other tests

Hemoglobin is also used to calculate other red blood cell indices, so it is often ordered together with related counts, such as hematocrit, rather than measured by itself.

Order online and a physician order is included — no doctor's visit and no insurance needed. Your sample is collected at a Quest Diagnostics patient service center.

Laboratory results should always be interpreted by a licensed clinician who knows your health history. This page is information about a test, not medical advice.

Frequently asked questions

What does the hemoglobin test measure?

It measures the amount of hemoglobin, the oxygen-carrying protein in red blood cells, in a whole blood sample.

How is the sample collected?

Through a routine blood draw, collected in an EDTA (lavender-top) tube for whole blood testing.

Do I need to prepare for a hemoglobin test?

No preparation instructions are listed for this test, though people follow any instructions their ordering clinician provides.

What is hemoglobin also called?

Quest lists this test under the abbreviations HB and Hgb.

Why is hemoglobin commonly tested?

It is commonly used as part of the evaluation for anemia and to calculate other red blood cell indices.

How much is a Hemoglobin test?

$5, paid once at checkout. The price includes the physician's order this test requires and sample collection at a Quest patient service center.

Last updated 2026-09-18 · How we write about lab testing

Educational content

This page is for general educational purposes only. It is not medical advice, and it is not a substitute for consultation with a qualified healthcare professional. Laboratory results should always be interpreted by a licensed clinician who knows your health history. If you have questions about your health or your results, talk with your doctor. If you think you may be experiencing a medical emergency, call 911.